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primary antibodies against p irs 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibodies against p irs 2
    Primary Antibodies Against P Irs 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+irs+2/IRS-2+Antibody/pm39796627-86-1-20
    Average 95 stars, based on 314 article reviews
    primary antibodies against p irs 2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism
    Article Snippet: The supernatants were collected and the concentration of each protein was determined using the PierceTM BCA protein assay kit (Thermo Scientific, Carlsbad, CA, USA). .. The proteins mixed with 4x loading buffer (20 μg/lane) were separated using 10% sodium dodecyl sulfate polyacrylamide gel and transferred to polyvinylidene difluoride membranes, which were further incubated for 1 h with primary antibodies against PPARγ, P-IRS-2, phospho-IRS2 (P-IRS-2), phospho-PI3K (P-PI3K), phosphor-Akt (P-Akt) (Ser473), Akt, PDX-1, and GAPDH (Cell Signaling, Danvers, MA, USA) at room temperature, and incubated again for 1 h with horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibodies (Cell Signaling, Boston, MA, USA) at room temperature [ ]. .. The proteins (ECL Advance, GE Healthcare, UK) were visualized using a chemiluminescence system (FUSION Solo, PEQLAB Biotechnologie GmbH, Erlangen, Germany) according to the manufacturer’s instructions.

    Article Title: IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1 −/− Mice Mediated by miR-33
    Article Snippet: Then each sample was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore Corporation, USA). .. The membranes were incubated with specific antibodies to IRS-1 (Cell Signaling Technology, USA), IRS-2, phosphorylated tyrosine (pTyr)-IRS-1, p-IRS-2 (USA), PI3K p85 antibody, AKT, p-AKT (Thr308) (Cell Signaling), β-actin (Abcam), or GAPDH (Santa Cruz), and then incubated with appropriate HRP-conjugated secondary antibodies. .. Blots were developed using an ECL Kit (Santa Cruz), and exposed to X-ray films.

    Article Title: Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic β-cell Metabolism.
    Article Snippet: The supernatants were collected and the concentration of each protein was determined using the PierceTM BCA protein assay kit (Thermo Scientific, Carlsbad, CA, USA). .. The proteins mixed with 4x loading buffer (20 μg/lane) were separated using 10% sodium dodecyl sulfate polyacrylamide gel and transferred to polyvinylidene difluoride membranes, which were further incubated for 1 h with primary antibodies against PPARγ, P-IRS-2, phospho-IRS2 (P-IRS-2), phospho-PI3K (P-PI3K), phosphor-Akt (P-Akt) (Ser473), Akt, PDX-1, and GAPDH (Cell Signaling, Danvers, MA, USA) at room temperature, and incubated again for 1 h with horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibodies (Cell Signaling, Boston, MA, USA) at room temperature [27]. .. The proteins (ECL Advance, GE Healthcare, UK) were visualized using a chemiluminescence system (FUSION Solo, PEQLAB Biotechnologie GmbH, Erlangen, Germany) according to the manufacturer’s instructions.

    Bicinchoninic Acid Protein Assay:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    ATP Assay:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    Lysis:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    Glucose Assay:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    Modification:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    MTT Assay:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).

    Saline:

    Article Title: Lycium ruthenicum Murray anthocyanins effectively inhibit α-glucosidase activity and alleviate insulin resistance
    Article Snippet: Reducing blood glucose level is an important strategy to control type 2 diabetes mellitus (T2DM).. This study aimed to explore the potential of Lycium ruthenicum Murray (LM) anthocyanins to reduce blood glucose levels.. LM anthocyanins effectively inhibited the activity of α-glucosidase of Caco-2 cells (IC50 value was 25.3 μg/mL), and promoted glucose consumption and uptake of Hep-G2 cells with insulin resistance (IR).



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